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siRNA Id: | aspsirna0932
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siRNA Name: | HWT-G93A-266
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | CUGCUGACAAAGAUGGUGU
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Mutant allele/target (5'->3'): | CUGCUGACAAAGAUGCUGU
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Target Mutation: | c.281G>C (p.Gly94Ala)
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Wild siRNA (As strand): | GACGACUGUUUCUACCACA
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ASP-siRNA (As strand): | GACGACUGUUUCUACGACA
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Mismatch position in siRNA: | G4C |
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Mutant allele (5'->3'): | CUGCUGACAAAGAUGCUGU
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ASP-siRNA (As strand): | GACGACUGUUUCUACGACA
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ASP-siRNA efficacy for mutant allele: | 85
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Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
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ASP-siRNA (As strand): | GACGACUGUUUCUACGACA
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ASP-siRNA efficacy for wild allele: | 90
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Percentage discrimination for ASP-siRNA: | -5
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Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
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Wild siRNA (As strand): | GACGACUGUUUCUACCACA
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Wild sirna efficacy for wild allele: | 30
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Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
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ASP-siRNA (As strand): | GACGACUGUUUCUACGACA
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Wild sirna efficacy for mutant allele: | 85
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Percentage discrimination for wild siRNA: | -55
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| Concentration: | 0.03microg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | GACTTGGGCAATGTGACTGCTGACAAAGAUGGUGUGGCCGAU |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 24/48/72 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | 14981234 |
