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siRNA Id: | aspsirna0935
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siRNA Name: | P-SUPER K-RAS V12
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| Gene: | K-RAS (KRAS) |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | GUUGGAGCUGGUGGCGUAG
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Mutant allele/target (5'->3'): | GUUGGAGCUGUUGGCGUAG
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Target Mutation: | c.35G>T,p.Gly12Val
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Wild siRNA (As strand): | CAACCUCGACCACCGCAUC
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ASP-siRNA (As strand): | CAACCUCGACAACCGCAUC
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Mismatch position in siRNA: | A9C |
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Mutant allele (5'->3'): | GUUGGAGCUGUUGGCGUAG
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ASP-siRNA (As strand): | CAACCUCGACAACCGCAUC
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
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ASP-siRNA (As strand): | CAACCUCGACAACCGCAUC
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
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Wild siRNA (As strand): | CAACCUCGACCACCGCAUC
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Wild sirna efficacy for wild allele: | 50
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Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
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ASP-siRNA (As strand): | CAACCUCGACAACCGCAUC
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Wild sirna efficacy for mutant allele: | 29
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Percentage discrimination for wild siRNA: | 21
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| Concentration: | 0.03microg |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P01116 |
| Target sequence: | GTTGGAGCTGTTGGCGTAG |
| Genbank Accesion: | NM_033360.2 |
| Cell-line: | CAPAN-1 |
| Experimental technique used: | Western blot |
| Transfection method: | Polybrene |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 211980 |
| KEGG disease ID: | H00013 |
| Reference: | 12242156 |
