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siRNA Id: | aspsirna0942
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siRNA Name: | Cag27 81 nt
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| Gene: | Androgen receptor (AR) |
| Disease: | Spinal and Bulbar Muscular Atrophy, X-linked 1; SMAX1/Kennedy’s disease |
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Wild allele/target (5'->3'): | AUGUCCUUCCUGCAGCAGC
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Mutant allele/target (5'->3'): | AUGGCCUUCCUGCAGCAGC
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Target Mutation: | c.172_174CAG(10_36) (p.Gln69_Gln80del)]
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Wild siRNA (As strand): | UACAGGAAGGACGUCGUCG
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ASP-siRNA (As strand): | UACCGGAAGGACGUCGUCG
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Mismatch position in siRNA: | C16A |
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Mutant allele (5'->3'): | AUGGCCUUCCUGCAGCAGC
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ASP-siRNA (As strand): | UACCGGAAGGACGUCGUCG
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ASP-siRNA efficacy for mutant allele: | 77
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Wild allele (5'->3'): | AUGUCCUUCCUGCAGCAGC
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ASP-siRNA (As strand): | UACCGGAAGGACGUCGUCG
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ASP-siRNA efficacy for wild allele: | 12
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Percentage discrimination for ASP-siRNA: | 65
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Wild allele (5'->3'): | AUGUCCUUCCUGCAGCAGC
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Wild siRNA (As strand): | UACAGGAAGGACGUCGUCG
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Wild sirna efficacy for wild allele: | Moderately effective
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Wild allele (5'->3'): | AUGUCCUUCCUGCAGCAGC
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ASP-siRNA (As strand): | UACCGGAAGGACGUCGUCG
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Wild sirna efficacy for mutant allele: | Low efficacy
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 1mIcrog |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P10275 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAG |
| Genbank Accesion: | NA |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 72 hours |
| OMID ID: | 313200 |
| KEGG disease ID: | H00062 |
| Reference: | 11809726 |
