aspsirna0951 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0951
siRNA Name: K12-Arg135Thr-4
Gene:Keratin 12 (KRT12)
Disease:Meesmann epithelial corneal dystrophy (MECD)
Wild allele/target (5'->3'): AAAAUCUUAAUGAUAGAUU
Mutant allele/target (5'->3'):AAAAUCUUAAUGAUACAUU
Target Mutation: c.404G>C,p.Arg135Thr
Wild siRNA (As strand):UUUUAGAAUUACUAUCUAA
ASP-siRNA (As strand):UUUUAGAAUUACUAUGUAA
Mismatch position in siRNA: G4C
Mutant allele (5'->3'): AAAAUCUUAAUGAUACAUU
ASP-siRNA (As strand):UUUUAGAAUUACUAUGUAA
ASP-siRNA efficacy for mutant allele:10
Wild allele (5'->3'): AAAAUCUUAAUGAUAGAUU
ASP-siRNA (As strand):UUUUAGAAUUACUAUGUAA
ASP-siRNA efficacy for wild allele:20
Percentage discrimination for ASP-siRNA:-10
Wild allele (5'->3'): AAAAUCUUAAUGAUAGAUU
Wild siRNA (As strand):UUUUAGAAUUACUAUCUAA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): AAAAUCUUAAUGAUAGAUU
ASP-siRNA (As strand):UUUUAGAAUUACUAUGUAA
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:0.25nM, 5nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q99456
Target sequence: GAAAAAGAAACTATGCAAAATCTTAATGATAG
Genbank Accesion: NM_000223.3
Cell-line: AD293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: MWG Biotech AG
Post-transfection duration: 72 hours
OMID ID: 122100
KEGG disease ID:H00726
Reference:23233254

.
.
.
.
.
.
.
.
.
.
.

.

.

.