aspsirna0963 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0963
siRNA Name: K12-Arg135Thr-16
Gene:Keratin 12 (KRT12)
Disease:Meesmann epithelial corneal dystrophy (MECD)
Wild allele/target (5'->3'): AUAGAUUAGCUUCCUACCU
Mutant allele/target (5'->3'):AUACAUUAGCUUCCUACCU
Target Mutation: c.404G>C,p.Arg135Thr
Wild siRNA (As strand):UAUCUAAUCGAAGGAUGGA
ASP-siRNA (As strand):UAUGUAAUCGAAGGAUGGA
Mismatch position in siRNA: G16C
Mutant allele (5'->3'): AUACAUUAGCUUCCUACCU
ASP-siRNA (As strand):UAUGUAAUCGAAGGAUGGA
ASP-siRNA efficacy for mutant allele:10
Wild allele (5'->3'): AUAGAUUAGCUUCCUACCU
ASP-siRNA (As strand):UAUGUAAUCGAAGGAUGGA
ASP-siRNA efficacy for wild allele:25
Percentage discrimination for ASP-siRNA:-15
Wild allele (5'->3'): AUAGAUUAGCUUCCUACCU
Wild siRNA (As strand):UAUCUAAUCGAAGGAUGGA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): AUAGAUUAGCUUCCUACCU
ASP-siRNA (As strand):UAUGUAAUCGAAGGAUGGA
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:0.25nM, 5nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q99456
Target sequence: GAAAAAGAAACTATGCAAAATCTTAATGATAG
Genbank Accesion: NM_000223.3
Cell-line: AD293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: MWG Biotech AG
Post-transfection duration: 72 hours
OMID ID: 122100
KEGG disease ID:H00726
Reference:23233254

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