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siRNA Id: | aspsirna0963
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siRNA Name: | K12-Arg135Thr-16
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Gene: | Keratin 12 (KRT12) |
Disease: | Meesmann epithelial corneal dystrophy (MECD) |
Wild allele/target (5'->3'): | AUAGAUUAGCUUCCUACCU
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Mutant allele/target (5'->3'): | AUACAUUAGCUUCCUACCU
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Target Mutation: | c.404G>C,p.Arg135Thr
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Wild siRNA (As strand): | UAUCUAAUCGAAGGAUGGA
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ASP-siRNA (As strand): | UAUGUAAUCGAAGGAUGGA
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Mismatch position in siRNA: | G16C |
Mutant allele (5'->3'): | AUACAUUAGCUUCCUACCU
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ASP-siRNA (As strand): | UAUGUAAUCGAAGGAUGGA
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ASP-siRNA efficacy for mutant allele: | 10
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Wild allele (5'->3'): | AUAGAUUAGCUUCCUACCU
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ASP-siRNA (As strand): | UAUGUAAUCGAAGGAUGGA
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ASP-siRNA efficacy for wild allele: | 25
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Percentage discrimination for ASP-siRNA: | -15
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Wild allele (5'->3'): | AUAGAUUAGCUUCCUACCU
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Wild siRNA (As strand): | UAUCUAAUCGAAGGAUGGA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | AUAGAUUAGCUUCCUACCU
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ASP-siRNA (As strand): | UAUGUAAUCGAAGGAUGGA
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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Concentration: | 0.25nM, 5nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | Q99456 |
Target sequence: | GAAAAAGAAACTATGCAAAATCTTAATGATAG |
Genbank Accesion: | NM_000223.3 |
Cell-line: | AD293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | MWG Biotech AG |
Post-transfection duration: | 72 hours |
OMID ID: | 122100 |
KEGG disease ID: | H00726 |
Reference: | 23233254 |