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siRNA Id: | aspsirna0970
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siRNA Name: | siHUNT2
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | NA
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Mutant allele/target (5'->3'): | GGCCUUCGAGUCCCUCAAGUCC
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | NA
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ASP-siRNA (As strand): | CCGGAAGCUCAGGGAGUUCAGG
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | GGCCUUCGAGUCCCUCAAGUCC
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ASP-siRNA (As strand): | CCGGAAGCUCAGGGAGUUCAGG
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | CCGGAAGCUCAGGGAGUUCAGG
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ASP-siRNA efficacy for wild allele: | 48
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Percentage discrimination for ASP-siRNA: | 32
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Wild allele (5'->3'): | NA
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Wild siRNA (As strand): | NA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | CCGGAAGCUCAGGGAGUUCAGG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 10mg |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | GGTGCTGAGCGGCGCCGCGAGTCGGCCCGAGG |
| Genbank Accesion: | L12392.1 |
| Cell-line: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 16019264 |
