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siRNA Id: | aspsirna0972
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siRNA Name: | d7
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| Gene: | Ataxin-1 (ATXN1) |
| Disease: | Spino Cerebellar Ataxia type-1 (SCA-1) |
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Wild allele/target (5'->3'): | NA
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Mutant allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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Target Mutation: | c.589_591CAG(36_38)
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Wild siRNA (As strand): | NA
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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ASP-siRNA efficacy for mutant allele: | 50
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Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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ASP-siRNA efficacy for wild allele: | 39
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Percentage discrimination for ASP-siRNA: | 11
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Wild allele (5'->3'): | NA
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Wild siRNA (As strand): | NA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P0C7T5 |
| Target sequence: | AGGCTGAGCAGCAGCAGCAGCAGCAGCAGC |
| Genbank Accesion: | NM_001128164.1 |
| Cell-line: | Fibroblast |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Metabion |
| Post-transfection duration: | 24 hours |
| OMID ID: | 64400 |
| KEGG disease ID: | H00056 |
| Reference: | 21427085 |
