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siRNA Id: | aspsirna0990
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siRNA Name: | W1/19
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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Mutant allele/target (5'->3'): | UAGCAGCAGCAGCAGCAGUAG
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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ASP-siRNA (As strand): | AUCGUCGUCGUCGUCGUCAUC
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Mismatch position in siRNA: | A3G,A21G |
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Mutant allele (5'->3'): | UAGCAGCAGCAGCAGCAGUAG
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ASP-siRNA (As strand): | AUCGUCGUCGUCGUCGUCAUC
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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ASP-siRNA (As strand): | AUCGUCGUCGUCGUCGUCAUC
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ASP-siRNA efficacy for wild allele: | 45
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Percentage discrimination for ASP-siRNA: | 15
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUC
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Wild sirna efficacy for wild allele: | 75
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAG
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ASP-siRNA (As strand): | AUCGUCGUCGUCGUCGUCAUC
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Wild sirna efficacy for mutant allele: | 60
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Percentage discrimination for wild siRNA: | 15
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| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P50914 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC |
| Genbank Accesion: | L12392.1 |
| Cell-line: | GM09197 |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Metabion |
| Post-transfection duration: | 24 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 21427085 |
