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siRNA Id: | aspsirna0999
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siRNA Name: | HAT DsRNA
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| Gene: | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1(CITED1) |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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Mutant allele/target (5'->3'): | GUGCGGCGGCGGCGCGGCGCG
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Target Mutation: | c.211A>G
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Wild siRNA (As strand): | CACGUCGCCGCCGCGCCGCGC
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ASP-siRNA (As strand): | CACGCCGCCGCCGCGCCGCGC
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Mismatch position in siRNA: | C17U |
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Mutant allele (5'->3'): | GUGCGGCGGCGGCGCGGCGCG
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ASP-siRNA (As strand): | CACGCCGCCGCCGCGCCGCGC
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ASP-siRNA efficacy for mutant allele: | 68
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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ASP-siRNA (As strand): | CACGCCGCCGCCGCGCCGCGC
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ASP-siRNA efficacy for wild allele: | 38
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Percentage discrimination for ASP-siRNA: | 30
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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Wild siRNA (As strand): | CACGUCGCCGCCGCGCCGCGC
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Wild sirna efficacy for wild allele: | Lowest effective
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Wild allele (5'->3'): | GUGCAGCGGCGGCGCGGCGCG
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ASP-siRNA (As strand): | CACGCCGCCGCCGCGCCGCGC
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Wild sirna efficacy for mutant allele: | 68
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 10mM |
| Orientation: | NA |
| Mismatch in: | siRNA |
| UniProt ID: | P34545 |
| Target sequence: | GGCACTGTGCGGUGCAGCGGCGGCGCGGCGCGGTGCGGG |
| Genbank Accesion: | NM_004143.3 |
| Cell-line: | N2 wild-type worms (C.Elegans) |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Promega |
| Post-transfection duration: | 24 hours |
| OMID ID: | 600140 |
| KEGG disease ID: | H00001 |
| Reference: | 11751575 |
