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siRNA Id: | aspsirna1013
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siRNA Name: | siRNAT2
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| Gene: | Collagen type I alpha 1 (COL1A1) |
| Disease: | Osteogenesis Imperfecta (OI) |
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Wild allele/target (5'->3'): | CCCCAAAAGCCAAAAAAUG
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Mutant allele/target (5'->3'): | CCUCAAAAGCCAAAAAAUG
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Target Mutation: | c.*88T>C
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Wild siRNA (As strand): | GGGGUUUUCGGUUUUUUAC
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ASP-siRNA (As strand): | GGAGUUUUCGGUUUUUUAC
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Mismatch position in siRNA: | A17G |
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Mutant allele (5'->3'): | CCUCAAAAGCCAAAAAAUG
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ASP-siRNA (As strand): | GGAGUUUUCGGUUUUUUAC
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ASP-siRNA efficacy for mutant allele: | 78
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Wild allele (5'->3'): | CCCCAAAAGCCAAAAAAUG
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ASP-siRNA (As strand): | GGAGUUUUCGGUUUUUUAC
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ASP-siRNA efficacy for wild allele: | 44
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Percentage discrimination for ASP-siRNA: | 34
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Wild allele (5'->3'): | CCCCAAAAGCCAAAAAAUG
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Wild siRNA (As strand): | GGGGUUUUCGGUUUUUUAC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CCCCAAAAGCCAAAAAAUG
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ASP-siRNA (As strand): | GGAGUUUUCGGUUUUUUAC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 50nMol |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P02452 |
| Target sequence: | CCCCTCAAAAGCCAAAAAATG |
| Genbank Accesion: | NM_000088.3 |
| Cell-line: | Cos-7 |
| Experimental technique used: | ELISA assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 166210 |
| KEGG disease ID: | H00506 |
| Reference: | 15241481 |
