| |
|---|
|
siRNA Id: | aspsirna1015
|
|
siRNA Name: | siRNAT1
|
| Gene: | Collagen type I alpha 1 (COL1A1) |
| Disease: | Osteogenesis Imperfecta (OI) |
|
Wild allele/target (5'->3'): | CUGAACCCCCCCAAAAGCC
|
|
Mutant allele/target (5'->3'): | CUGAACCCCCUCAAAAGCC
|
|
Target Mutation: | c.*88T>C
|
|
Wild siRNA (As strand): | GACUUGGGGGGGUUUUCGG
|
|
ASP-siRNA (As strand): | GACUUGGGGGAGUUUUCGG
|
|
Mismatch position in siRNA: | A11G |
|
Mutant allele (5'->3'): | CUGAACCCCCUCAAAAGCC
|
|
ASP-siRNA (As strand): | GACUUGGGGGAGUUUUCGG
|
|
ASP-siRNA efficacy for mutant allele: | 75
|
|
Wild allele (5'->3'): | CUGAACCCCCCCAAAAGCC
|
|
ASP-siRNA (As strand): | GACUUGGGGGAGUUUUCGG
|
|
ASP-siRNA efficacy for wild allele: | 64
|
|
Percentage discrimination for ASP-siRNA: | 11
|
|
Wild allele (5'->3'): | CUGAACCCCCCCAAAAGCC
|
|
Wild siRNA (As strand): | GACUUGGGGGGGUUUUCGG
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | CUGAACCCCCCCAAAAGCC
|
|
ASP-siRNA (As strand): | GACUUGGGGGAGUUUUCGG
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 50nMol |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P02452 |
| Target sequence: | AACTGAACCCCCTCAAAAGCC |
| Genbank Accesion: | NM_000088.3 |
| Cell-line: | Mesenchymal progenitor Stem cells(MPCs) |
| Experimental technique used: | ELISA assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 166210 |
| KEGG disease ID: | H00506 |
| Reference: | 15241481 |
