aspsirna1018 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1018
siRNA Name: L1 siRNA
Gene:Cu,Zn superoxide dismutase (SOD1)
Disease:Amyotrophic Lateral Sclerosis (ALS)
Wild allele/target (5'->3'): GAGACUUGGGCAAUGUGAC
Mutant allele/target (5'->3'):UAGACUUGGGCAAUGUGAC
Target Mutation: c.256G>C,p.Gly86Arg
Wild siRNA (As strand):CUCUGAACCCGUUACACUG
ASP-siRNA (As strand):CUCUGAACCCGUUACACUG
Mismatch position in siRNA: U1G(S)
Mutant allele (5'->3'): UAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand):CUCUGAACCCGUUACACUG
ASP-siRNA efficacy for mutant allele:75
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand):CUCUGAACCCGUUACACUG
ASP-siRNA efficacy for wild allele:10
Percentage discrimination for ASP-siRNA:65
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
Wild siRNA (As strand):CUCUGAACCCGUUACACUG
Wild sirna efficacy for wild allele:8
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand):CUCUGAACCCGUUACACUG
Wild sirna efficacy for mutant allele:80
Percentage discrimination for wild siRNA:72
Concentration:2.0microg/mL
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P00441
Target sequence: AGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAA
Genbank Accesion: NM_000454.4
Cell-line: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
OMID ID: 105400
KEGG disease ID:H00058
Reference:18215350

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