| |
|---|
|
siRNA Id: | aspsirna1029
|
|
siRNA Name: | R1+R2
|
| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
|
Wild allele/target (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
Mutant allele/target (5'->3'): | GAGACUUGGGCAAUGUGUC
|
|
Target Mutation: | c.256G>C,p.Gly86Arg
|
|
Wild siRNA (As strand): | CUCUGAACCCGUUACACUG?
|
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
|
Mismatch position in siRNA: | U1G(S),U18A(S) |
|
Mutant allele (5'->3'): | GAGACUUGGGCAAUGUGUC
|
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
|
ASP-siRNA efficacy for mutant allele: | 80
|
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
|
ASP-siRNA efficacy for wild allele: | 58
|
|
Percentage discrimination for ASP-siRNA: | 22
|
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
Wild siRNA (As strand): | CUCUGAACCCGUUACACUG?
|
|
Wild sirna efficacy for wild allele: | 8
|
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
|
Wild sirna efficacy for mutant allele: | 80
|
|
Percentage discrimination for wild siRNA: | 72
|
| Concentration: | 2.0microg/mL |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | AGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAA |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | 18215350 |
