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siRNA Id: | aspsirna1031
|
siRNA Name: | R1+R4
|
Gene: | Cu,Zn superoxide dismutase (SOD1) |
Disease: | Amyotrophic Lateral Sclerosis (ALS) |
Wild allele/target (5'->3'): | GAGACUUGGGCAAUGUGAC
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Mutant allele/target (5'->3'): | GAGACUUGGGCAAUGAGAC
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Target Mutation: | c.256G>C,p.Gly86Arg
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Wild siRNA (As strand): | CUCUGAACCCGUUACACUG?
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
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Mismatch position in siRNA: | U1G(S),A16U(S) |
Mutant allele (5'->3'): | GAGACUUGGGCAAUGAGAC
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
ASP-siRNA efficacy for mutant allele: | 45
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
ASP-siRNA efficacy for wild allele: | 45
|
Percentage discrimination for ASP-siRNA: | 0
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
Wild siRNA (As strand): | CUCUGAACCCGUUACACUG?
|
Wild sirna efficacy for wild allele: | 8
|
Wild allele (5'->3'): | GAGACUUGGGCAAUGUGAC
|
ASP-siRNA (As strand): | CUCUGAACCCGUUACACUU
|
Wild sirna efficacy for mutant allele: | 80
|
Percentage discrimination for wild siRNA: | 72
|
Concentration: | 2.0microg/mL |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | P00441 |
Target sequence: | AGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAA |
Genbank Accesion: | NM_000454.4 |
Cell-line: | HEK293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 24 hours |
OMID ID: | 105400 |
KEGG disease ID: | H00058 |
Reference: | 18215350 |