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siRNA Id: | aspsirna1115
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siRNA Name: | siCD46-14A18C
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| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
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Wild allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
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Mutant allele/target (5'->3'): | CGUAUUGGAGAGAGCACGA
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Target Mutation: | NM_002389.4:c.104G>A, p.Cys35Tyr
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Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA (As strand): | GCAUAACCUCUCUCGUGCU
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Mismatch position in siRNA: | 14A,18C |
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Mutant allele (5'->3'): | CGUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand): | GCAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for mutant allele: | 18
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand): | GCAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for wild allele: | 40
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Percentage discrimination for ASP-siRNA: | -22
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand): | GCAUAACCUCUCUCGUGCU
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Wild sirna efficacy for mutant allele: | 38
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | Target gene |
| UniProt ID: | P15529 |
| Target sequence: | CTTATTGGAGAGAGCACGATTTATTGTGGTGACA |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 18420656 |
