| |
|---|
|
siRNA Id: | aspsirna1661
|
|
siRNA Name: | siCD46-5G12T
|
| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
|
Wild allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
Mutant allele/target (5'->3'): | CUUAUUGAAGAGAGCACGA
|
|
Target Mutation: | NM_002389.4:c.104G>A, p.Cys35Tyr
|
|
Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
ASP-siRNA (As strand): | GAAUAACUUCUCUCGUGCU
|
|
Mismatch position in siRNA: | 5G,12U |
|
Mutant allele (5'->3'): | CUUAUUGAAGAGAGCACGA
|
|
ASP-siRNA (As strand): | GAAUAACUUCUCUCGUGCU
|
|
ASP-siRNA efficacy for mutant allele: | 58
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand): | GAAUAACUUCUCUCGUGCU
|
|
ASP-siRNA efficacy for wild allele: | 38
|
|
Percentage discrimination for ASP-siRNA: | 20
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand): | GAAUAACUUCUCUCGUGCU
|
|
Wild sirna efficacy for mutant allele: | 38
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | Target gene |
| UniProt ID: | P15529 |
| Target sequence: | CTTATTGGAGAGAGCACGATTTATTGTGGTGACA |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 18420656 |
