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siRNA Id: | aspsirna1751
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siRNA Name: | BetaS(G:U)-siRNA
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| Gene: | Hb beta-chain (HBB) |
| Disease: | Sickle cell anemia |
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Wild allele/target (5'->3'): | UGACUCCUGAGGAGAAGUC
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Mutant allele/target (5'->3'): | UGACUCCUGUGGAGAAGUU
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Target Mutation: | c.365A>T (p.Glu122Val)
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Wild siRNA (As strand): | ACUGAGGACUCCUCUUCAG
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ASP-siRNA (As strand): | ACUGAGGACACCUCUUCAG
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Mismatch position in siRNA: | A10C,G1U |
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Mutant allele (5'->3'): | UGACUCCUGUGGAGAAGUU
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ASP-siRNA (As strand): | ACUGAGGACACCUCUUCAG
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | UGACUCCUGAGGAGAAGUC
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ASP-siRNA (As strand): | ACUGAGGACACCUCUUCAG
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ASP-siRNA efficacy for wild allele: | 5
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | UGACUCCUGAGGAGAAGUC
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Wild siRNA (As strand): | ACUGAGGACUCCUCUUCAG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | UGACUCCUGAGGAGAAGUC
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ASP-siRNA (As strand): | ACUGAGGACACCUCUUCAG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 200nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P68871 |
| Target sequence: | ATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCC |
| Genbank Accesion: | NM_000518.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 603903 |
| KEGG disease ID: | H00229 |
| Reference: | 16585504 |
