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siRNA Id: | aspsirna1768
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siRNA Name: | La21-3'm2m12
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| Gene: | P. luciferase |
| Disease: | NA |
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Wild allele/target (5'->3'): | ACCGCUGGAGAGCAACUGC
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Mutant allele/target (5'->3'): | UGCUGAGAGGAUCAGCAUU
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Target Mutation: | NA
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Wild siRNA (As strand): | UGGCGACCUCUCGUUGACG
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ASP-siRNA (As strand): | ACGACUCUCCUAGUCGUAA
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Mismatch position in siRNA: | A1G,A2C,A8U |
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Mutant allele (5'->3'): | UGCUGAGAGGAUCAGCAUU
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ASP-siRNA (As strand): | ACGACUCUCCUAGUCGUAA
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | ACCGCUGGAGAGCAACUGC
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ASP-siRNA (As strand): | ACGACUCUCCUAGUCGUAA
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | ACCGCUGGAGAGCAACUGC
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Wild siRNA (As strand): | UGGCGACCUCUCGUUGACG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | ACCGCUGGAGAGCAACUGC
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ASP-siRNA (As strand): | ACGACUCUCCUAGUCGUAA
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Wild sirna efficacy for mutant allele: | 98
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 40nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P08659 |
| Target sequence: | GCUCAACGAGAGGUCGCCA |
| Genbank Accesion: | M15077.1 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Proligo |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 14741366 |
