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siRNA Id: | aspsirna1770
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siRNA Name: | Lam-3'm2m12
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Gene: | Human lamin A/C (LMNA) |
Disease: | NA |
Wild allele/target (5'->3'): | UGCUGAGAGGAACAGCAACCU
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Mutant allele/target (5'->3'): | UGCUGAGUGGAACAGCAUU
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Target Mutation: | NA
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Wild siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
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ASP-siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
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Mismatch position in siRNA: | A1C,A2U,A8C |
Mutant allele (5'->3'): | UGCUGAGUGGAACAGCAUU
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ASP-siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | UGCUGAGAGGAACAGCAACCU
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ASP-siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | UGCUGAGAGGAACAGCAACCU
|
Wild siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
|
Wild sirna efficacy for wild allele: | NA
|
Wild allele (5'->3'): | UGCUGAGAGGAACAGCAACCU
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ASP-siRNA (As strand): | AGACGACUCUCCUUGUCGUUG
|
Wild sirna efficacy for mutant allele: | NA
|
Percentage discrimination for wild siRNA: | 70
|
Concentration: | 100nM |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | P02545 |
Target sequence: | UGCUGAGAGGAACAGCAAC |
Genbank Accesion: | NM_170707.3 |
Cell-line: | HeLa |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | JetSI transfection reagent |
siRNA expression method: | Proligo |
Post-transfection duration: | 48 hours |
OMID ID: | NA |
KEGG disease ID: | NA |
Reference: | 14741366 |