aspsirna1770 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1770
siRNA Name: Lam-3'm2m12
Gene:Human lamin A/C (LMNA)
Disease:NA
Wild allele/target (5'->3'): UGCUGAGAGGAACAGCAACCU
Mutant allele/target (5'->3'):UGCUGAGUGGAACAGCAUU
Target Mutation: NA
Wild siRNA (As strand):AGACGACUCUCCUUGUCGUUG
ASP-siRNA (As strand):AGACGACUCUCCUUGUCGUUG
Mismatch position in siRNA: A1C,A2U,A8C
Mutant allele (5'->3'): UGCUGAGUGGAACAGCAUU
ASP-siRNA (As strand):AGACGACUCUCCUUGUCGUUG
ASP-siRNA efficacy for mutant allele:NA
Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
ASP-siRNA (As strand):AGACGACUCUCCUUGUCGUUG
ASP-siRNA efficacy for wild allele:NA
Percentage discrimination for ASP-siRNA:NA
Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
Wild siRNA (As strand):AGACGACUCUCCUUGUCGUUG
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): UGCUGAGAGGAACAGCAACCU
ASP-siRNA (As strand):AGACGACUCUCCUUGUCGUUG
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:70
Concentration:100nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P02545
Target sequence: UGCUGAGAGGAACAGCAAC
Genbank Accesion: NM_170707.3
Cell-line: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: JetSI transfection reagent
siRNA expression method: Proligo
Post-transfection duration: 48 hours
OMID ID: NA
KEGG disease ID:NA
Reference:14741366

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