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siRNA Id: | aspsirna1774
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siRNA Name: | Dn1(#1)-3'm2m12
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| Gene: | DNA (cytosine-5-)-methyltransferase 1 (DNMT1) |
| Disease: | NA |
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Wild allele/target (5'->3'): | GUCCGCAGGCGGCUCAAAGAUU
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Mutant allele/target (5'->3'): | GUCCGCAGUCGGCUCAAAUU
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Target Mutation: | NA
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Wild siRNA (As strand): | CAGGCGUCCGCCGAGUUUCUAA
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ASP-siRNA (As strand): | CAGGCGUCCGCCGAGUUUCU
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Mismatch position in siRNA: | A1U,A2U,A8U |
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Mutant allele (5'->3'): | GUCCGCAGUCGGCUCAAAUU
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ASP-siRNA (As strand): | CAGGCGUCCGCCGAGUUUCU
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | GUCCGCAGGCGGCUCAAAGAUU
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ASP-siRNA (As strand): | CAGGCGUCCGCCGAGUUUCU
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | GUCCGCAGGCGGCUCAAAGAUU
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Wild siRNA (As strand): | CAGGCGUCCGCCGAGUUUCUAA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GUCCGCAGGCGGCUCAAAGAUU
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ASP-siRNA (As strand): | CAGGCGUCCGCCGAGUUUCU
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | 90
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| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P26358 |
| Target sequence: | CTGCCCGACGATGTCCGCAGGCGGCTCAAAGATT |
| Genbank Accesion: | NM_001379 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | JetSI transfection reagent |
| siRNA expression method: | Proligo |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 14741366 |
