Purple | = Wild vs mutant target sequence along with mutation information |
Brown | = Wild vs ASP-siRNA description along with positional differnece between two |
Light pink | = ASP siRNA efficacy for mutant vs wild allele |
Green | = Wild siRNA efficacy for Wild vs Mutant allele |
siRNA Id: | aspsirna1777 |
siRNA Name: | si10C |
Gene: | Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) |
Disease: | Cancer |
Wild allele/target (5'->3'): | GCUGCGCUAUGGCGAAGAC |
Mutant allele/target (5'->3'): | GCUGCGCUACGGCGAAGAC |
Target Mutation: | c.2907C>T,(p.Ile969=) |
Wild siRNA (As strand): | CGACGCGAUACCGCUUCUG |
ASP-siRNA (As strand): | CGACGCGAUGCCGCUUCUG |
Mismatch position in siRNA: | G10A |
Mutant allele (5'->3'): | GCUGCGCUACGGCGAAGAC |
ASP-siRNA (As strand): | CGACGCGAUGCCGCUUCUG |
ASP-siRNA efficacy for mutant allele: | NA |
Wild allele (5'->3'): | GCUGCGCUAUGGCGAAGAC |
ASP-siRNA (As strand): | CGACGCGAUGCCGCUUCUG |
ASP-siRNA efficacy for wild allele: | 20 |
Percentage discrimination for ASP-siRNA: | NA |
Wild allele (5'->3'): | GCUGCGCUAUGGCGAAGAC |
Wild siRNA (As strand): | CGACGCGAUACCGCUUCUG |
Wild sirna efficacy for wild allele: | NA |
Wild allele (5'->3'): | GCUGCGCUAUGGCGAAGAC |
ASP-siRNA (As strand): | CGACGCGAUGCCGCUUCUG |
Wild sirna efficacy for mutant allele: | NA |
Percentage discrimination for wild siRNA: | NA |
Concentration: | 100nM |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | P24928 |
Target sequence: | TTCCACGCCATGGGGGGTCGTGAGGGGCTCATTGACA |
Genbank Accesion: | NM_000937.4 |
Cell-line: | MiaPaca II |
Experimental technique used: | Northern blot |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Proligo |
Post-transfection duration: | 24 hours |
OMID ID: | 180660 |
KEGG disease ID: | H00014 |
Reference: | 19165236 |
siRNA Id: | aspsirna1777 |
siRNA Name: | siCD46 |
Gene: | Complement regulatory protein (CD46) |
Disease: | Hemolytic uremic syndrome |
Wild allele/target (5'->3'): | NA |
Mutant allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA |
Target Mutation: | c.104G>A, p.Cys35Tyr |
Wild siRNA (As strand): | NA |
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
Mismatch position in siRNA: | G:C pair |
Mutant allele (5'->3'): | CUUAUUGGAGAGAGCACGA |
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
ASP-siRNA efficacy for mutant allele: | 93 |
Wild allele (5'->3'): | NA |
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
ASP-siRNA efficacy for wild allele: | NA |
Percentage discrimination for ASP-siRNA: | NA |
Wild allele (5'->3'): | NA |
Wild siRNA (As strand): | NA |
Wild sirna efficacy for wild allele: | NA |
Wild allele (5'->3'): | NA |
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
Wild sirna efficacy for mutant allele: | 93 |
Percentage discrimination for wild siRNA: | NA |
Concentration: | 13nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P15529 |
Target sequence: | GCCCCCAATATGTGAAAAG |
Genbank Accesion: | NM_002389.4 |
Cell-line: | HEK293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 24 hours |
OMID ID: | 120920 |
KEGG disease ID: | H00106, H0027 |
Reference: | 15781493 |