| |
siRNA Id: | aspsirna1800
|
siRNA Name: | D1
|
Gene: | keratin 6a (KRT6a) |
Disease: | Pachyonychia congenita (PC) |
Wild allele/target (5'->3'): | UCAAGACCCUCAACAACAAGUU
|
Mutant allele/target (5'->3'): | UCAAGACCCUC---AACAAGUU
|
Target Mutation: | c.514_516delAAC,p.Asn172del
|
Wild siRNA (As strand): | AGUUCUGGGAGUUGUUGUUCAA
|
ASP-siRNA (As strand): | AGUUCUGGGAG---UUGUUCAA
|
Mismatch position in siRNA: | 9,10,11 deletion |
Mutant allele (5'->3'): | UCAAGACCCUC---AACAAGUU
|
ASP-siRNA (As strand): | AGUUCUGGGAG---UUGUUCAA
|
ASP-siRNA efficacy for mutant allele: | 75
|
Wild allele (5'->3'): | UCAAGACCCUCAACAACAAGUU
|
ASP-siRNA (As strand): | AGUUCUGGGAG---UUGUUCAA
|
ASP-siRNA efficacy for wild allele: | 20
|
Percentage discrimination for ASP-siRNA: | 55
|
Wild allele (5'->3'): | UCAAGACCCUCAACAACAAGUU
|
Wild siRNA (As strand): | AGUUCUGGGAGUUGUUGUUCAA
|
Wild sirna efficacy for wild allele: | NA
|
Wild allele (5'->3'): | UCAAGACCCUCAACAACAAGUU
|
ASP-siRNA (As strand): | AGUUCUGGGAG---UUGUUCAA
|
Wild sirna efficacy for mutant allele: | NA
|
Percentage discrimination for wild siRNA: | NA
|
Concentration: | 800ng |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P02538 |
Target sequence: | AGCGUGAACAGAUCAAGACCCUCAACAACAAGUUUGCCUCCUUCA |
Genbank Accesion: | NM_005554.3 |
Cell-line: | 293 FT |
Experimental technique used: | Flourescence microscopy |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Qiagen |
Post-transfection duration: | 24 hours |
OMID ID: | 167200 |
KEGG disease ID: | H00684 |
Reference: | 17145926 |
| |
siRNA Id: | aspsirna1800
|
siRNA Name: | siCD46
|
Gene: | Complement regulatory protein (CD46) |
Disease: | Hemolytic uremic syndrome |
Wild allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
|
Mutant allele/target (5'->3'): | CUUAUCGGAGAGAGCACGA
|
Target Mutation: | c.104G>A, p.Cys35Tyr
|
Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
Mismatch position in siRNA: | A:C clash |
Mutant allele (5'->3'): | CUUAUCGGAGAGAGCACGA
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
ASP-siRNA efficacy for mutant allele: | 43
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
ASP-siRNA efficacy for wild allele: | 93
|
Percentage discrimination for ASP-siRNA: | 50
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
Wild sirna efficacy for wild allele: | 93
|
Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
Wild sirna efficacy for mutant allele: | 43
|
Percentage discrimination for wild siRNA: | 50
|
Concentration: | 13nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P15529 |
Target sequence: | GCCCCCAATATGTGAAAAG |
Genbank Accesion: | NM_002389.4 |
Cell-line: | HEK293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 24 hours |
OMID ID: | 120920 |
KEGG disease ID: | H00106, H0027 |
Reference: | 15781493 |
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