aspsirna1800 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1800
siRNA Name: D1
Gene:keratin 6a (KRT6a)
Disease:Pachyonychia congenita (PC)
Wild allele/target (5'->3'): UCAAGACCCUCAACAACAAGUU
Mutant allele/target (5'->3'):UCAAGACCCUC---AACAAGUU
Target Mutation: c.514_516delAAC,p.Asn172del
Wild siRNA (As strand):AGUUCUGGGAGUUGUUGUUCAA
ASP-siRNA (As strand):AGUUCUGGGAG---UUGUUCAA
Mismatch position in siRNA: 9,10,11 deletion
Mutant allele (5'->3'): UCAAGACCCUC---AACAAGUU
ASP-siRNA (As strand):AGUUCUGGGAG---UUGUUCAA
ASP-siRNA efficacy for mutant allele:75
Wild allele (5'->3'): UCAAGACCCUCAACAACAAGUU
ASP-siRNA (As strand):AGUUCUGGGAG---UUGUUCAA
ASP-siRNA efficacy for wild allele:20
Percentage discrimination for ASP-siRNA:55
Wild allele (5'->3'): UCAAGACCCUCAACAACAAGUU
Wild siRNA (As strand):AGUUCUGGGAGUUGUUGUUCAA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): UCAAGACCCUCAACAACAAGUU
ASP-siRNA (As strand):AGUUCUGGGAG---UUGUUCAA
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:800ng
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P02538
Target sequence: AGCGUGAACAGAUCAAGACCCUCAACAACAAGUUUGCCUCCUUCA
Genbank Accesion: NM_005554.3
Cell-line: 293 FT
Experimental technique used: Flourescence microscopy
Transfection method: Lipofectamine 2000
siRNA expression method: Qiagen
Post-transfection duration: 24 hours
OMID ID: 167200
KEGG disease ID:H00684
Reference:17145926
siRNA Id:aspsirna1800
siRNA Name: siCD46
Gene:Complement regulatory protein (CD46)
Disease:Hemolytic uremic syndrome
Wild allele/target (5'->3'): CUUAUUGGAGAGAGCACGA
Mutant allele/target (5'->3'):CUUAUCGGAGAGAGCACGA
Target Mutation: c.104G>A, p.Cys35Tyr
Wild siRNA (As strand):GAAUAACCUCUCUCGUGCU
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
Mismatch position in siRNA: A:C clash
Mutant allele (5'->3'): CUUAUCGGAGAGAGCACGA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
ASP-siRNA efficacy for mutant allele:43
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
ASP-siRNA efficacy for wild allele:93
Percentage discrimination for ASP-siRNA:50
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
Wild siRNA (As strand):GAAUAACCUCUCUCGUGCU
Wild sirna efficacy for wild allele:93
Wild allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
Wild sirna efficacy for mutant allele:43
Percentage discrimination for wild siRNA:50
Concentration:13nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P15529
Target sequence: GCCCCCAATATGTGAAAAG
Genbank Accesion: NM_002389.4
Cell-line: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
OMID ID: 120920
KEGG disease ID:H00106, H0027
Reference:15781493

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