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siRNA Id: | aspsirna1801
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siRNA Name: | D2
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| Gene: | keratin 6a (KRT6a) |
| Disease: | Pachyonychia congenita (PC) |
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Wild allele/target (5'->3'): | CAAGACCCUCAACAACAAGUUU
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Mutant allele/target (5'->3'): | CAAGACCCUC---AACAAGUUU
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Target Mutation: | c.514_516delAAC,p.Asn172del
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Wild siRNA (As strand): | GUUCUGGGAGUUGUUGUUCAAA
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ASP-siRNA (As strand): | GUUCUGGGAG---UUGUUCAAA
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Mismatch position in siRNA: | 10,11,12 deletion |
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Mutant allele (5'->3'): | CAAGACCCUC---AACAAGUUU
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ASP-siRNA (As strand): | GUUCUGGGAG---UUGUUCAAA
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ASP-siRNA efficacy for mutant allele: | 30
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Wild allele (5'->3'): | CAAGACCCUCAACAACAAGUUU
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ASP-siRNA (As strand): | GUUCUGGGAG---UUGUUCAAA
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 10
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Wild allele (5'->3'): | CAAGACCCUCAACAACAAGUUU
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Wild siRNA (As strand): | GUUCUGGGAGUUGUUGUUCAAA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CAAGACCCUCAACAACAAGUUU
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ASP-siRNA (As strand): | GUUCUGGGAG---UUGUUCAAA
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 800ng |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02538 |
| Target sequence: | AGCGUGAACAGAUCAAGACCCUCAACAACAAGUUUGCCUCCUUCA |
| Genbank Accesion: | NM_005554.3 |
| Cell-line: | 293 FT |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 167200 |
| KEGG disease ID: | H00684 |
| Reference: | 17145926 |
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siRNA Id: | aspsirna1801
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siRNA Name: | siCD46
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| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
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Wild allele/target (5'->3'): | NA
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Mutant allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
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Target Mutation: | c.104G>A, p.Cys35Tyr
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Wild siRNA (As strand): | NA
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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Mismatch position in siRNA: | C:G pair |
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Mutant allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for mutant allele: | 93
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Wild allele (5'->3'): | NA
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for wild allele: | NA
|
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Percentage discrimination for ASP-siRNA: | NA
|
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Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand): | NA
|
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Wild sirna efficacy for wild allele: | NA
|
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Wild allele (5'->3'): | NA
|
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
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Wild sirna efficacy for mutant allele: | 93
|
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Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P15529 |
| Target sequence: | GCCCCCAATATGTGAAAAG |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 15781493 |
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