| |
|---|
|
siRNA Id: | aspsirna1809
|
|
siRNA Name: | s10
|
| Gene: | Coagulation factor III (thromboplastin, tissue factor) (F3) /CD142 |
| Disease: | Malignant melanoma |
|
Wild allele/target (5'->3'): | GCGCUUCAGGCACUACAAAUA
|
|
Mutant allele/target (5'->3'): | GCGCUUCAGCCACUACAAAUA
|
|
Target Mutation: | c.489G>A,p.Arg163=
|
|
Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU
|
|
ASP-siRNA (As strand): | CGCGAAGUCGGUGAUGUUUAU
|
|
Mismatch position in siRNA: | G12C |
|
Mutant allele (5'->3'): | GCGCUUCAGCCACUACAAAUA
|
|
ASP-siRNA (As strand): | CGCGAAGUCGGUGAUGUUUAU
|
|
ASP-siRNA efficacy for mutant allele: | NA
|
|
Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
|
|
ASP-siRNA (As strand): | CGCGAAGUCGGUGAUGUUUAU
|
|
ASP-siRNA efficacy for wild allele: | NA
|
|
Percentage discrimination for ASP-siRNA: | NA
|
|
Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
|
|
Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
|
|
ASP-siRNA (As strand): | CGCGAAGUCGGUGAUGUUUAU
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q99081 |
| Target sequence: | AGGTGGCCGGCGCTTCAGGCACTACAAATACTGTGG |
| Genbank Accesion: | NM_001178096.1 |
| Cell-line: | HaCaT |
| Experimental technique used: | Northern blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Glen Research,PerSeptive Biosystems |
| Post-transfection duration: | 48 hours |
| OMID ID: | 134390 |
| KEGG disease ID: | NA |
| Reference: | 12527766 |
| |
|---|
|
siRNA Id: | aspsirna1809
|
|
siRNA Name: | siCD46
|
| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
|
Wild allele/target (5'->3'): | NA
|
|
Mutant allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
Target Mutation: | c.104G>A, p.Cys35Tyr
|
|
Wild siRNA (As strand): | NA
|
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
Mismatch position in siRNA: | U:A pair |
|
Mutant allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
ASP-siRNA efficacy for mutant allele: | 93
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
ASP-siRNA efficacy for wild allele: | NA
|
|
Percentage discrimination for ASP-siRNA: | NA
|
|
Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand): | NA
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
|
Wild sirna efficacy for mutant allele: | 93
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P15529 |
| Target sequence: | GCCCCCAATATGTGAAAAG |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 15781493 |
.
.
.
.
.
.
.
.
.
.
.
.
.
.
