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siRNA Id: | aspsirna1811
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siRNA Name: | s13
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| Gene: | Coagulation factor III (thromboplastin, tissue factor) (F3) /CD142 |
| Disease: | Malignant melanoma |
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Wild allele/target (5'->3'): | GCGCUUCAGGCACUACAAAUA
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Mutant allele/target (5'->3'): | GCGCUUCAGGCAGUACAAAUA
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Target Mutation: | c.489G>A,p.Arg163=
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Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU
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ASP-siRNA (As strand): | CGCGAAGUCCGUCAUGUUUAU
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Mismatch position in siRNA: | C9G |
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Mutant allele (5'->3'): | GCGCUUCAGGCAGUACAAAUA
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ASP-siRNA (As strand): | CGCGAAGUCCGUCAUGUUUAU
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
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ASP-siRNA (As strand): | CGCGAAGUCCGUCAUGUUUAU
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
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Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA
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ASP-siRNA (As strand): | CGCGAAGUCCGUCAUGUUUAU
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q99081 |
| Target sequence: | AGGTGGCCGGCGCTTCAGGCACTACAAATACTGTGG |
| Genbank Accesion: | NM_001178096.1 |
| Cell-line: | HaCaT |
| Experimental technique used: | Northern blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Glen Research,PerSeptive Biosystems |
| Post-transfection duration: | 48 hours |
| OMID ID: | 134390 |
| KEGG disease ID: | NA |
| Reference: | 12527766 |
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siRNA Id: | aspsirna1811
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|
siRNA Name: | siCD46
|
| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
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Wild allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA
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Mutant allele/target (5'->3'): | CUUAUUGGUGAGAGCACGA
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Target Mutation: | c.104G>A, p.Cys35Tyr
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Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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Mismatch position in siRNA: | U:U clash |
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Mutant allele (5'->3'): | CUUAUUGGUGAGAGCACGA
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for mutant allele: | 15
|
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
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ASP-siRNA efficacy for wild allele: | 93
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Percentage discrimination for ASP-siRNA: | 78
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
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Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
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Wild sirna efficacy for wild allele: | 93
|
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Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA
|
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ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU
|
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Wild sirna efficacy for mutant allele: | 15
|
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Percentage discrimination for wild siRNA: | 78
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| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P15529 |
| Target sequence: | GCCCCCAATATGTGAAAAG |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 15781493 |
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