| Purple | = Wild vs mutant target sequence along with mutation information |
| Brown | = Wild vs ASP-siRNA description along with positional differnece between two |
| Light pink | = ASP siRNA efficacy for mutant vs wild allele |
| Green | = Wild siRNA efficacy for Wild vs Mutant allele |
| siRNA Id: | aspsirna1816 |
| siRNA Name: | Ds10/16 |
| Gene: | Coagulation factor III (thromboplastin, tissue factor) (F3) /CD142 |
| Disease: | Malignant melanoma |
| Wild allele/target (5'->3'): | GCGCUUCAGGCACUACAAAUA |
| Mutant allele/target (5'->3'): | GCGCUUCAGCCACUAGAAAUA |
| Target Mutation: | c.489G>A,p.Arg163= |
| Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU |
| ASP-siRNA (As strand): | CGCGAAGUCGGUGAUCUUUAU |
| Mismatch position in siRNA: | G12C,C6G |
| Mutant allele (5'->3'): | GCGCUUCAGCCACUAGAAAUA |
| ASP-siRNA (As strand): | CGCGAAGUCGGUGAUCUUUAU |
| ASP-siRNA efficacy for mutant allele: | NA |
| Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA |
| ASP-siRNA (As strand): | CGCGAAGUCGGUGAUCUUUAU |
| ASP-siRNA efficacy for wild allele: | Poor efficacy |
| Percentage discrimination for ASP-siRNA: | NA |
| Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA |
| Wild siRNA (As strand): | CGCGAAGUCCGUGAUGUUUAU |
| Wild sirna efficacy for wild allele: | NA |
| Wild allele (5'->3'): | GCGCUUCAGGCACUACAAAUA |
| ASP-siRNA (As strand): | CGCGAAGUCGGUGAUCUUUAU |
| Wild sirna efficacy for mutant allele: | NA |
| Percentage discrimination for wild siRNA: | NA |
| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q99081 |
| Target sequence: | GCGCUUCAGCCACUAGAAAUA |
| Genbank Accesion: | NM_001178096.1 |
| Cell-line: | HaCaT |
| Experimental technique used: | Northern blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Glen Research,PerSeptive Biosystems |
| Post-transfection duration: | 48 hours |
| OMID ID: | 134390 |
| KEGG disease ID: | NA |
| Reference: | 12527766 |
| siRNA Id: | aspsirna1816 |
| siRNA Name: | siCD46 |
| Gene: | Complement regulatory protein (CD46) |
| Disease: | Hemolytic uremic syndrome |
| Wild allele/target (5'->3'): | CUUAUUGGAGAGAGCACGA |
| Mutant allele/target (5'->3'): | CUUAUUGGACAGAGCACGA |
| Target Mutation: | c.104G>A, p.Cys35Tyr |
| Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| Mismatch position in siRNA: | C:C clash |
| Mutant allele (5'->3'): | CUUAUUGGACAGAGCACGA |
| ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| ASP-siRNA efficacy for mutant allele: | 2 |
| Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA |
| ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| ASP-siRNA efficacy for wild allele: | 93 |
| Percentage discrimination for ASP-siRNA: | 91 |
| Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA |
| Wild siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| Wild sirna efficacy for wild allele: | 93 |
| Wild allele (5'->3'): | CUUAUUGGAGAGAGCACGA |
| ASP-siRNA (As strand): | GAAUAACCUCUCUCGUGCU |
| Wild sirna efficacy for mutant allele: | 2 |
| Percentage discrimination for wild siRNA: | 91 |
| Concentration: | 13nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P15529 |
| Target sequence: | GCCCCCAATATGTGAAAAG |
| Genbank Accesion: | NM_002389.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 120920 |
| KEGG disease ID: | H00106, H0027 |
| Reference: | 15781493 |