aspsirna1841 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1841
siRNA Name: RMC466 w
Gene:Aquaporin 4 (Aqp4)
Disease:Brain edema
Wild allele/target (5'->3'): UGGCCAUGGGCUCCUGGUGGA
Mutant allele/target (5'->3'):UGGCCAUGGGCUCCUGGUAGA
Target Mutation: NA
Wild siRNA (As strand):ACCGGUACCCGAGGACCACCU
ASP-siRNA (As strand):ACCGGUACCCGAGGACCAUCU
Mismatch position in siRNA: NA
Mutant allele (5'->3'): UGGCCAUGGGCUCCUGGUAGA
ASP-siRNA (As strand):ACCGGUACCCGAGGACCAUCU
ASP-siRNA efficacy for mutant allele:10
Wild allele (5'->3'): UGGCCAUGGGCUCCUGGUGGA
ASP-siRNA (As strand):ACCGGUACCCGAGGACCAUCU
ASP-siRNA efficacy for wild allele:42
Percentage discrimination for ASP-siRNA:-32
Wild allele (5'->3'): UGGCCAUGGGCUCCUGGUGGA
Wild siRNA (As strand):ACCGGUACCCGAGGACCACCU
Wild sirna efficacy for wild allele:35
Wild allele (5'->3'): UGGCCAUGGGCUCCUGGUGGA
ASP-siRNA (As strand):ACCGGUACCCGAGGACCAUCU
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:1.44mg
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P55087
Target sequence: UGGCCAUGGGCUCCUGGUAGA
Genbank Accesion: NM_009700
Cell-line: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Ambion
Post-transfection duration: 24 hours
OMID ID: 600308
KEGG disease ID:H00106
Reference:16113241
siRNA Id:aspsirna1841
siRNA Name: siCD46
Gene:Complement regulatory protein (CD46)
Disease:Hemolytic uremic syndrome
Wild allele/target (5'->3'): NA
Mutant allele/target (5'->3'):CUUAUUGGAGAGAGCACGA
Target Mutation: c.104G>A, p.Cys35Tyr
Wild siRNA (As strand):NA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
Mismatch position in siRNA: G:C pair
Mutant allele (5'->3'): CUUAUUGGAGAGAGCACGA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
ASP-siRNA efficacy for mutant allele:93
Wild allele (5'->3'): NA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
ASP-siRNA efficacy for wild allele:NA
Percentage discrimination for ASP-siRNA:NA
Wild allele (5'->3'): NA
Wild siRNA (As strand):NA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand):GAAUAACCUCUCUCGUGCU
Wild sirna efficacy for mutant allele:93
Percentage discrimination for wild siRNA:NA
Concentration:13nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P15529
Target sequence: GCCCCCAATATGTGAAAAG
Genbank Accesion: NM_002389.4
Cell-line: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 24 hours
OMID ID: 120920
KEGG disease ID:H00106, H0027
Reference:15781493

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