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siRNA Id: | aspsirna1862
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siRNA Name: | RMC845 m
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| Gene: | Aquaporin 4 (Aqp4) |
| Disease: | Brain edema |
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Wild allele/target (5'->3'): | AACCGGAGCCAAGUGGAGACA
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Mutant allele/target (5'->3'): | AACCGGAGCCAAGUGGAGCCA
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Target Mutation: | NA
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Wild siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
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ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCGGU
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | AACCGGAGCCAAGUGGAGCCA
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ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCGGU
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ASP-siRNA efficacy for mutant allele: | 88
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Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
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ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCGGU
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ASP-siRNA efficacy for wild allele: | 85
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Percentage discrimination for ASP-siRNA: | 3
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Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
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Wild siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
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Wild sirna efficacy for wild allele: | 58
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Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
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ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCGGU
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Wild sirna efficacy for mutant allele: | 75
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Percentage discrimination for wild siRNA: | -17
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| Concentration: | 1.44mg |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P55087 |
| Target sequence: | AACCGGAGCCAAGUGGAGCCA |
| Genbank Accesion: | NM_009700 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 24 hours |
| OMID ID: | 600308 |
| KEGG disease ID: | H00106 |
| Reference: | 16113241 |
