| |
|---|
|
siRNA Id: | aspsirna1863
|
|
siRNA Name: | RMC845 mm
|
| Gene: | Aquaporin 4 (Aqp4) |
| Disease: | Brain edema |
|
Wild allele/target (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
Mutant allele/target (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
Target Mutation: | NA
|
|
Wild siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
Mismatch position in siRNA: | NA |
|
Mutant allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
ASP-siRNA efficacy for mutant allele: | 93
|
|
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
ASP-siRNA efficacy for wild allele: | 82
|
|
Percentage discrimination for ASP-siRNA: | 11
|
|
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
Wild siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
Wild sirna efficacy for wild allele: | 58
|
|
Wild allele (5'->3'): | AACCGGAGCCAAGUGGAGACA
|
|
ASP-siRNA (As strand): | UUGGCCUCGGUUCACCUCUGU
|
|
Wild sirna efficacy for mutant allele: | 75
|
|
Percentage discrimination for wild siRNA: | -17
|
| Concentration: | 1.44mg |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P55087 |
| Target sequence: | AACCGGAGCCAAGUGGAGACA |
| Genbank Accesion: | NM_009700 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 24 hours |
| OMID ID: | 600308 |
| KEGG disease ID: | H00106 |
| Reference: | 16113241 |
