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siRNA Id: | aspsirna1877
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siRNA Name: | RMC821 w
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Gene: | Aquaporin 4 (Aqp4) |
Disease: | Brain edema |
Wild allele/target (5'->3'): | GGGAGCUACAUGGAGGUGGAG
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Mutant allele/target (5'->3'): | GGGAGCUACAUGGAGGUGAAG
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Target Mutation: | NA
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Wild siRNA (As strand): | CCCUCGAUGUACCUCCACCUC
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ASP-siRNA (As strand): | CCCUCGAUGUACCUCCACUUC
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Mismatch position in siRNA: | NA |
Mutant allele (5'->3'): | GGGAGCUACAUGGAGGUGAAG
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ASP-siRNA (As strand): | CCCUCGAUGUACCUCCACUUC
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | GGGAGCUACAUGGAGGUGGAG
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ASP-siRNA (As strand): | CCCUCGAUGUACCUCCACUUC
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ASP-siRNA efficacy for wild allele: | 55
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Percentage discrimination for ASP-siRNA: | 25
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Wild allele (5'->3'): | GGGAGCUACAUGGAGGUGGAG
|
Wild siRNA (As strand): | CCCUCGAUGUACCUCCACCUC
|
Wild sirna efficacy for wild allele: | 75
|
Wild allele (5'->3'): | GGGAGCUACAUGGAGGUGGAG
|
ASP-siRNA (As strand): | CCCUCGAUGUACCUCCACUUC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
|
Concentration: | 1.44mg |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | P55087 |
Target sequence: | GGGAGCUACAUGGAGGUGAAG |
Genbank Accesion: | NM_009700 |
Cell-line: | HeLa |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Ambion |
Post-transfection duration: | 24 hours |
OMID ID: | 600308 |
KEGG disease ID: | H00106 |
Reference: | 16113241 |