aspsirna1880 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1880
siRNA Name: RMC869 w
Gene:Aquaporin 4 (Aqp4)
Disease:Brain edema
Wild allele/target (5'->3'): GACUUGAUCCUGAAGCCCGGG
Mutant allele/target (5'->3'):GACUUGAUCCUGAAGCCCAGG
Target Mutation: NA
Wild siRNA (As strand):CUGAACUAGGACUUCGGGCCC
ASP-siRNA (As strand):CUGAACUAGGACUUCGGGUCC
Mismatch position in siRNA: NA
Mutant allele (5'->3'): GACUUGAUCCUGAAGCCCAGG
ASP-siRNA (As strand):CUGAACUAGGACUUCGGGUCC
ASP-siRNA efficacy for mutant allele:70
Wild allele (5'->3'): GACUUGAUCCUGAAGCCCGGG
ASP-siRNA (As strand):CUGAACUAGGACUUCGGGUCC
ASP-siRNA efficacy for wild allele:83
Percentage discrimination for ASP-siRNA:-13
Wild allele (5'->3'): GACUUGAUCCUGAAGCCCGGG
Wild siRNA (As strand):CUGAACUAGGACUUCGGGCCC
Wild sirna efficacy for wild allele:82
Wild allele (5'->3'): GACUUGAUCCUGAAGCCCGGG
ASP-siRNA (As strand):CUGAACUAGGACUUCGGGUCC
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:1.44mg
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P55087
Target sequence: GACUUGAUCCUGAAGCCCAGG
Genbank Accesion: NM_009700
Cell-line: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Ambion
Post-transfection duration: 24 hours
OMID ID: 600308
KEGG disease ID:H00106
Reference:16113241

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