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siRNA Id: | aspsirna1889
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siRNA Name: | d-Mut
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| Gene: | Collagen type I alpha 1 (COL1A1) |
| Disease: | Osteogenesis Imperfecta (OI) |
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Wild allele/target (5'->3'): | CCUGGUGAAGCUGGUCGUC
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Mutant allele/target (5'->3'): | CCUGGUGAAGCUUGUCGUC
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Target Mutation: | c.3244G>T,p.Gly1082Cys
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Wild siRNA (As strand): | GGACCACUUCGACCAGCAG
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ASP-siRNA (As strand): | GGACCACUUCGAACAGCAG
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Mismatch position in siRNA: | A7C |
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Mutant allele (5'->3'): | CCUGGUGAAGCUUGUCGUC
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ASP-siRNA (As strand): | GGACCACUUCGAACAGCAG
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ASP-siRNA efficacy for mutant allele: | 47
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Wild allele (5'->3'): | CCUGGUGAAGCUGGUCGUC
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ASP-siRNA (As strand): | GGACCACUUCGAACAGCAG
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ASP-siRNA efficacy for wild allele: | 18
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Percentage discrimination for ASP-siRNA: | 29
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Wild allele (5'->3'): | CCUGGUGAAGCUGGUCGUC
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Wild siRNA (As strand): | GGACCACUUCGACCAGCAG
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Wild sirna efficacy for wild allele: | 80
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Wild allele (5'->3'): | CCUGGUGAAGCUGGUCGUC
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ASP-siRNA (As strand): | GGACCACUUCGAACAGCAG
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Wild sirna efficacy for mutant allele: | 35
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Percentage discrimination for wild siRNA: | 45
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| Concentration: | 10nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P02452 |
| Target sequence: | TCTCCTGGTGAAGCTGGTCGTCCCGGTGAAGCTGGTCTGCCTGGTGC |
| Genbank Accesion: | NM_000088.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Eurogentec |
| Post-transfection duration: | 24 hours |
| OMID ID: | 615220 |
| KEGG disease ID: | H00506 |
| Reference: | 24022296 |
