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siRNA Id: | aspsirna1909
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siRNA Name: | 403m (14)
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| Gene: | Myosin Heavy Chain6 (Myh6) |
| Disease: | Human hypertrophic cardiomyopathy (HCM) |
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Wild allele/target (5'->3'): | CCCUCGGGUGAGGGUGGGGAC
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Mutant allele/target (5'->3'): | CCCUCAGGUGAGGGUGGGGAC
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Target Mutation: | c.2384G>A,p.Arg795Gln
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Wild siRNA (As strand): | GGGAGCCCACUCCCACCCCUG
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ASP-siRNA (As strand): | GGGAGUCCACUCCCACCCCUG
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Mismatch position in siRNA: | U16C |
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Mutant allele (5'->3'): | CCCUCAGGUGAGGGUGGGGAC
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ASP-siRNA (As strand): | GGGAGUCCACUCCCACCCCUG
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ASP-siRNA efficacy for mutant allele: | 78
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Wild allele (5'->3'): | CCCUCGGGUGAGGGUGGGGAC
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ASP-siRNA (As strand): | GGGAGUCCACUCCCACCCCUG
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ASP-siRNA efficacy for wild allele: | 70
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Percentage discrimination for ASP-siRNA: | 8
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Wild allele (5'->3'): | CCCUCGGGUGAGGGUGGGGAC
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Wild siRNA (As strand): | GGGAGCCCACUCCCACCCCUG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CCCUCGGGUGAGGGUGGGGAC
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ASP-siRNA (As strand): | GGGAGUCCACUCCCACCCCUG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 10nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P33076 |
| Target sequence: | AAGGGCCUGUGUCACCCUCGGGUGAGGGUGGGGACCGAGUAU |
| Genbank Accesion: | NM_002471.3 |
| Cell-line: | 293T |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 608758 |
| KEGG disease ID: | H00292 |
| Reference: | 24092743 |
