| |
|---|
|
siRNA Id: | aspsirna1922
|
|
siRNA Name: | si747/49_3D11
|
| Gene: | epidermal growth factor receptor (EGFR) |
| Disease: | Cancer |
|
Wild allele/target (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
Mutant allele/target (5'->3'): | UCAAGGAA----------CAACAUCUCC
|
|
Target Mutation: | c.2235_2243delGGAATTAAG, p.Leu747_Glu749del
|
|
Wild siRNA (As strand): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
|
|
ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
|
|
Mismatch position in siRNA: | DEL_12,13,14 |
|
Mutant allele (5'->3'): | UCAAGGAA----------CAACAUCUCC
|
|
ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
|
|
ASP-siRNA efficacy for mutant allele: | 60
|
|
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
|
|
ASP-siRNA efficacy for wild allele: | 5
|
|
Percentage discrimination for ASP-siRNA: | 55
|
|
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
Wild siRNA (As strand): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
|
|
Wild sirna efficacy for wild allele: | 59
|
|
Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
|
|
ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
|
|
Wild sirna efficacy for mutant allele: | 11
|
|
Percentage discrimination for wild siRNA: | 48
|
| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P00533 |
| Target sequence: | UCAAGGAACCAACAUCUCC |
| Genbank Accesion: | NM_005228.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Applied Biosystems |
| Post-transfection duration: | 24 hours |
| OMID ID: | 211980 |
| KEGG disease ID: | H00013 |
| Reference: | 23951344 |
