aspsirna1923 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1923
siRNA Name: si747/49_3D3
Gene:epidermal growth factor receptor (EGFR)
Disease:Cancer
Wild allele/target (5'->3'): CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
Mutant allele/target (5'->3'):CGUCGCUAUCAAGGAA----------CAA
Target Mutation: c.2248G>C,p.A750P
Wild siRNA (As strand):GCAGCGAUAGUUCCUUAAUUCUCUUCGUU
ASP-siRNA (As strand):GCAGCGAUAGUUCCUU----------GUU
Mismatch position in siRNA: DEL_4,5,6
Mutant allele (5'->3'): CGUCGCUAUCAAGGAA----------CAA
ASP-siRNA (As strand):GCAGCGAUAGUUCCUU----------GUU
ASP-siRNA efficacy for mutant allele:82
Wild allele (5'->3'): CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
ASP-siRNA (As strand):GCAGCGAUAGUUCCUU----------GUU
ASP-siRNA efficacy for wild allele:10
Percentage discrimination for ASP-siRNA:72
Wild allele (5'->3'): CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
Wild siRNA (As strand):GCAGCGAUAGUUCCUUAAUUCUCUUCGUU
Wild sirna efficacy for wild allele:59
Wild allele (5'->3'): CGUCGCUAUCAAGGAAUUAAGAGAAGCAA
ASP-siRNA (As strand):GCAGCGAUAGUUCCUU----------GUU
Wild sirna efficacy for mutant allele:11
Percentage discrimination for wild siRNA:48
Concentration:20nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P00533
Target sequence: CGUCGCUAUCAAGGAACCA
Genbank Accesion: NM_005228.3
Cell-line: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Applied Biosystems
Post-transfection duration: 24 hours
OMID ID: 211980
KEGG disease ID:H00013
Reference:23951344

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