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siRNA Id: | aspsirna1931
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siRNA Name: | si747/49_3D11
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| Gene: | epidermal growth factor receptor (EGFR) |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
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Mutant allele/target (5'->3'): | UCAAGGAA----------CAACAUCUCC
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Target Mutation: | c.2248G>C,p.A750P
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Wild siRNA (As strand): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
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ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
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Mismatch position in siRNA: | DEL_12,13,14 |
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Mutant allele (5'->3'): | UCAAGGAA----------CAACAUCUCC
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ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
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ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
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ASP-siRNA efficacy for wild allele: | 5
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Percentage discrimination for ASP-siRNA: | 55
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Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
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Wild siRNA (As strand): | AGUUCCUUAAUUCUCUUCGUUGUAGAGG
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Wild sirna efficacy for wild allele: | 59
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Wild allele (5'->3'): | UCAAGGAAUUAAGAGAAGCAACAUCUCC
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ASP-siRNA (As strand): | AGUUCCUU---GUUGUAGAGG
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Wild sirna efficacy for mutant allele: | 11
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Percentage discrimination for wild siRNA: | 48
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| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P00533 |
| Target sequence: | UCAAGGAACCAACAUCUCC |
| Genbank Accesion: | NM_005228.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Applied Biosystems |
| Post-transfection duration: | 24 hours |
| OMID ID: | 211980 |
| KEGG disease ID: | H00013 |
| Reference: | 23951344 |
