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siRNA Id: | aspsirna1948
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siRNA Name: | siRNA-4
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| Gene: | Collagen type VI alpha 3 (COL6A3) |
| Disease: | Dominant ullrich muscular dystrophy |
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Wild allele/target (5'->3'): | AAAGGGUGAGCGUGGUCCA
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Mutant allele/target (5'->3'): | AAAGGGUGAGCGUGGUCCG
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Target Mutation: | (IVS16DS plus 1G-A) in intron 16
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Wild siRNA (As strand): | UUUCCCACUCGCACCAGGU
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ASP-siRNA (As strand): | UUUCCCACUCGCACCAGGC
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Mismatch position in siRNA: | C1U |
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Mutant allele (5'->3'): | AAAGGGUGAGCGUGGUCCG
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ASP-siRNA (As strand): | UUUCCCACUCGCACCAGGC
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | AAAGGGUGAGCGUGGUCCA
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ASP-siRNA (As strand): | UUUCCCACUCGCACCAGGC
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | 75
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Wild allele (5'->3'): | AAAGGGUGAGCGUGGUCCA
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Wild siRNA (As strand): | UUUCCCACUCGCACCAGGU
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Wild sirna efficacy for wild allele: | 43
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Wild allele (5'->3'): | AAAGGGUGAGCGUGGUCCA
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ASP-siRNA (As strand): | UUUCCCACUCGCACCAGGC
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Wild sirna efficacy for mutant allele: | 12
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Percentage discrimination for wild siRNA: | 31
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| Concentration: | 5nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P12111 |
| Target sequence: | UCGGCAGCAUCGGGCCAAAGGGUAUUC |
| Genbank Accesion: | NM_057166.4 |
| Cell-line: | Dermal fibroblasts |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 254090 |
| KEGG disease ID: | H01341 |
| Reference: | 24518369 |
