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siRNA Id: | aspsirna1952
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siRNA Name: | siRNA-6
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Gene: | Collagen type VI alpha 3 (COL6A3) |
Disease: | Dominant ullrich muscular dystrophy |
Wild allele/target (5'->3'): | GGCCAAAGGGUGAGCGUGA
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Mutant allele/target (5'->3'): | GGCCAAAGGGUGAGCGUGG
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Target Mutation: | (IVS16DS plus 1G-A) in intron 16
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Wild siRNA (As strand): | CCGGUUUCCCACUCGCACU
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ASP-siRNA (As strand): | CCGGUUUCCCACUCGCACC
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Mismatch position in siRNA: | C1U |
Mutant allele (5'->3'): | GGCCAAAGGGUGAGCGUGG
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ASP-siRNA (As strand): | CCGGUUUCCCACUCGCACC
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | GGCCAAAGGGUGAGCGUGA
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ASP-siRNA (As strand): | CCGGUUUCCCACUCGCACC
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | 60
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Wild allele (5'->3'): | GGCCAAAGGGUGAGCGUGA
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Wild siRNA (As strand): | CCGGUUUCCCACUCGCACU
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Wild sirna efficacy for wild allele: | 43
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Wild allele (5'->3'): | GGCCAAAGGGUGAGCGUGA
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ASP-siRNA (As strand): | CCGGUUUCCCACUCGCACC
|
Wild sirna efficacy for mutant allele: | 12
|
Percentage discrimination for wild siRNA: | 31
|
Concentration: | 5nM |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | P12111 |
Target sequence: | UCGGCAGCAUCGGGCCAAAGGGUAUUC |
Genbank Accesion: | NM_057166.4 |
Cell-line: | Dermal fibroblasts |
Experimental technique used: | RT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Invitrogen |
Post-transfection duration: | 48 hours |
OMID ID: | 254090 |
KEGG disease ID: | H01341 |
Reference: | 24518369 |