aspsirna1960 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna1960
siRNA Name: siRNA-3
Gene:Collagen type VI alpha 3 (COL6A3)
Disease:Dominant ullrich muscular dystrophy
Wild allele/target (5'->3'): CAAAGGGUGAGCGUGGUCA
Mutant allele/target (5'->3'):CAAAGGGUGAGCGUGGUCC
Target Mutation: (IVS16DS plus 1G-A) in intron 16
Wild siRNA (As strand):GUUUCCCACUCGCACCAGU
ASP-siRNA (As strand):GUUUCCCACUCGCACCAGG
Mismatch position in siRNA: G1U
Mutant allele (5'->3'): CAAAGGGUGAGCGUGGUCC
ASP-siRNA (As strand):GUUUCCCACUCGCACCAGG
ASP-siRNA efficacy for mutant allele:NA
Wild allele (5'->3'): CAAAGGGUGAGCGUGGUCA
ASP-siRNA (As strand):GUUUCCCACUCGCACCAGG
ASP-siRNA efficacy for wild allele:NA
Percentage discrimination for ASP-siRNA:70
Wild allele (5'->3'): CAAAGGGUGAGCGUGGUCA
Wild siRNA (As strand):GUUUCCCACUCGCACCAGU
Wild sirna efficacy for wild allele:43
Wild allele (5'->3'): CAAAGGGUGAGCGUGGUCA
ASP-siRNA (As strand):GUUUCCCACUCGCACCAGG
Wild sirna efficacy for mutant allele:12
Percentage discrimination for wild siRNA:31
Concentration:5nM
Orientation:Double strand
Mismatch in:siRNA
UniProt ID: P12111
Target sequence: UCGGCAGCAUCGGGCCAAAGGGUAUUC
Genbank Accesion: NM_057166.4
Cell-line: HEK293
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 48 hours
OMID ID: 254090
KEGG disease ID:H01341
Reference:24518369

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