| |
|---|
|
siRNA Id: | aspsirna1971
|
|
siRNA Name: | siRNA-3
|
| Gene: | Collagen type VI alpha 3 (COL6A3) |
| Disease: | Congenital muscular dystrophy type Ullrich (UCMD) |
|
Wild allele/target (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
Mutant allele/target (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
Target Mutation: | (IVS16DS plus 1G-A) in intron 16
|
|
Wild siRNA (As strand): | GUUUCCCACUCGCACCAGU
|
|
ASP-siRNA (As strand): | GUUUCCCACUCGCACCAGG
|
|
Mismatch position in siRNA: | G1U |
|
Mutant allele (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
ASP-siRNA (As strand): | GUUUCCCACUCGCACCAGG
|
|
ASP-siRNA efficacy for mutant allele: | 40
|
|
Wild allele (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
ASP-siRNA (As strand): | GUUUCCCACUCGCACCAGG
|
|
ASP-siRNA efficacy for wild allele: | 30
|
|
Percentage discrimination for ASP-siRNA: | 10
|
|
Wild allele (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
Wild siRNA (As strand): | GUUUCCCACUCGCACCAGU
|
|
Wild sirna efficacy for wild allele: | 50
|
|
Wild allele (5'->3'): | CAAAGGGUGAGCGUGGUCA
|
|
ASP-siRNA (As strand): | GUUUCCCACUCGCACCAGG
|
|
Wild sirna efficacy for mutant allele: | 60
|
|
Percentage discrimination for wild siRNA: | -10
|
| Concentration: | 5nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P12111 |
| Target sequence: | UCGGCAGCAUCGGGCCAAAGGGUAUUC |
| Genbank Accesion: | NM_057166.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48-72 hours |
| OMID ID: | 254090 |
| KEGG disease ID: | H01341 |
| Reference: | 24518369 |
