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siRNA Id: | aspsirna1994
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siRNA Name: | Luc-5 siRNA-g5
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| Gene: | P. luciferase |
| Disease: | NA |
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Wild allele/target (5'->3'): | UUUAUGAGGAUCUCUCUGA
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Mutant allele/target (5'->3'): | UUUAUGAGGAUCUCCCUGA
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Target Mutation: | NA
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Wild siRNA (As strand): | AAAUACUCCUAGAGAGACU
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ASP-siRNA (As strand): | AAAUACUCCUAGAGGGACU
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Mismatch position in siRNA: | G5A |
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Mutant allele (5'->3'): | UUUAUGAGGAUCUCCCUGA
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ASP-siRNA (As strand): | AAAUACUCCUAGAGGGACU
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ASP-siRNA efficacy for mutant allele: | 81
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Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
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ASP-siRNA (As strand): | AAAUACUCCUAGAGGGACU
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 51
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Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
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Wild siRNA (As strand): | AAAUACUCCUAGAGAGACU
|
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Wild sirna efficacy for wild allele: | 30
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|
Wild allele (5'->3'): | UUUAUGAGGAUCUCUCUGA
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ASP-siRNA (As strand): | AAAUACUCCUAGAGGGACU
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 100nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P19840 |
| Target sequence: | CCCTTCTTGGCCTTTATGAGGATCTCTCTGATTT |
| Genbank Accesion: | KP171169.1 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | US2005/0176045A1 |
