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siRNA Id: | aspsirna2134
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siRNA Name: | 25
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| Gene: | K-RAS (KRAS) |
| Disease: | Cancer |
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Wild allele/target (5'->3'): | UGGGCGUAGGCAAGAGUGC
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Mutant allele/target (5'->3'): | UUGGCGUAGGCAAGAGUGC
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Target Mutation: | c.35G>T,p.Gly12Val
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Wild siRNA (As strand): | ACCCGCAUCCGUUCUCACG
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ASP-siRNA (As strand): | AACCGCAUCCGUUCUCACG
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Mismatch position in siRNA: | A18C |
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Mutant allele (5'->3'): | UUGGCGUAGGCAAGAGUGC
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ASP-siRNA (As strand): | AACCGCAUCCGUUCUCACG
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ASP-siRNA efficacy for mutant allele: | 3
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Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
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ASP-siRNA (As strand): | AACCGCAUCCGUUCUCACG
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ASP-siRNA efficacy for wild allele: | 15
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Percentage discrimination for ASP-siRNA: | -12
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Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
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Wild siRNA (As strand): | ACCCGCAUCCGUUCUCACG
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Wild sirna efficacy for wild allele: | 67
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Wild allele (5'->3'): | UGGGCGUAGGCAAGAGUGC
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ASP-siRNA (As strand): | AACCGCAUCCGUUCUCACG
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Wild sirna efficacy for mutant allele: | 23
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Percentage discrimination for wild siRNA: | 44
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| Concentration: | 100nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P01116 |
| Target sequence: | gaatataaacttgtggtagttggagctggtggcgtaggcaagagtgccttgacgatacagctaattcagaatcattt |
| Genbank Accesion: | NM_033360.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 190070 |
| KEGG disease ID: | H00003 |
| Reference: | US2005/0176045A1 |
