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siRNA Id: | aspsirna2167
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siRNA Name: | 618383
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | CAGUUCAGCUACAGCAGAA
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Mutant allele/target (5'->3'): | CAGUUCAGCUGCAGCAGAU-P
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | GUCAAGUCGAUGUCGUCUU
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ASP-siRNA (As strand): | GUCAAGUCGACGUCGUCUU-P
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Mismatch position in siRNA: | A9U |
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Mutant allele (5'->3'): | CAGUUCAGCUGCAGCAGAU-P
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ASP-siRNA (As strand): | GUCAAGUCGACGUCGUCUU-P
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ASP-siRNA efficacy for mutant allele: | 59
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Wild allele (5'->3'): | CAGUUCAGCUACAGCAGAA
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ASP-siRNA (As strand): | GUCAAGUCGACGUCGUCUU-P
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ASP-siRNA efficacy for wild allele: | 11
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Percentage discrimination for ASP-siRNA: | 48
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Wild allele (5'->3'): | CAGUUCAGCUACAGCAGAA
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Wild siRNA (As strand): | GUCAAGUCGAUGUCGUCUU
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CAGUUCAGCUACAGCAGAA
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ASP-siRNA (As strand): | GUCAAGUCGACGUCGUCUU-P
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 25nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P42858 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | GM04281/GM04869 |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Applied Biosystems |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 24694346 |
