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siRNA Id: | aspsirna2169
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siRNA Name: | K12-Leu140Arg-9
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| Gene: | Keratin 12 (KRT12) |
| Disease: | Meesmann epithelial corneal Dystrophy (MECD) |
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Wild allele/target (5'->3'): | AUGCAAAAUCAUAAUGAUA
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Mutant allele/target (5'->3'): | AUGCAAAAUCCUAAUGAUA
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Target Mutation: | c.419T>G,p.Leu140Arg
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Wild siRNA (As strand): | UACGUUUUAGUAUUACUAU
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ASP-siRNA (As strand): | UACGUUUUAGGAUUACUAU
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Mismatch position in siRNA: | G9U |
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Mutant allele (5'->3'): | AUGCAAAAUCCUAAUGAUA
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ASP-siRNA (As strand): | UACGUUUUAGGAUUACUAU
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ASP-siRNA efficacy for mutant allele: | 89
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Wild allele (5'->3'): | AUGCAAAAUCAUAAUGAUA
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ASP-siRNA (As strand): | UACGUUUUAGGAUUACUAU
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ASP-siRNA efficacy for wild allele: | 8
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Percentage discrimination for ASP-siRNA: | 81
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Wild allele (5'->3'): | AUGCAAAAUCAUAAUGAUA
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Wild siRNA (As strand): | UACGUUUUAGUAUUACUAU
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Wild sirna efficacy for wild allele: | 45
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Wild allele (5'->3'): | AUGCAAAAUCAUAAUGAUA
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ASP-siRNA (As strand): | UACGUUUUAGGAUUACUAU
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Wild sirna efficacy for mutant allele: | 60
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Percentage discrimination for wild siRNA: | -15
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| Concentration: | 5nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q99456 |
| Target sequence: | CCTAATGATAGATTAGCTTCCTACCTGG |
| Genbank Accesion: | NM_000223.3 |
| Cell-line: | AD293 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24/48/72hours |
| OMID ID: | 122100 |
| KEGG disease ID: | H00726 |
| Reference: | 24801514 |
