aspsirna2194 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna2194
siRNA Name: 8A–13A
Gene:Collagen type VI alpha 1 (COL6A1)
Disease:Ullrich congenital muscular dystrophy (UCMD),Bethlem myopathy (BM)
Wild allele/target (5'->3'): AGAAGCCGGAGAUCCUGGA
Mutant allele/target (5'->3'):AGAAGCCAGAGAACCUGGA
Target Mutation: c.850G>A, p.G284R
Wild siRNA (As strand):UCUUCGGCCUCUAGGACCU
ASP-siRNA (As strand):UCUUCGGUCUCUUGGACCU
Mismatch position in siRNA: U12C,U7A
Mutant allele (5'->3'): AGAAGCCAGAGAACCUGGA
ASP-siRNA (As strand):UCUUCGGUCUCUUGGACCU
ASP-siRNA efficacy for mutant allele:80
Wild allele (5'->3'): AGAAGCCGGAGAUCCUGGA
ASP-siRNA (As strand):UCUUCGGUCUCUUGGACCU
ASP-siRNA efficacy for wild allele:22
Percentage discrimination for ASP-siRNA:58
Wild allele (5'->3'): AGAAGCCGGAGAUCCUGGA
Wild siRNA (As strand):UCUUCGGCCUCUAGGACCU
Wild sirna efficacy for wild allele:80
Wild allele (5'->3'): AGAAGCCGGAGAUCCUGGA
ASP-siRNA (As strand):UCUUCGGUCUCUUGGACCU
Wild sirna efficacy for mutant allele:40
Percentage discrimination for wild siRNA:40
Concentration:80nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P12109
Target sequence: AGGGAGAAGCCGGAGAUCCUGGAA
Genbank Accesion: NM_001848.2
Cell-line: NIH3T3
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
OMID ID: 254090
KEGG disease ID:H01341
Reference:24959844

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