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siRNA Id: | aspsirna2195
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siRNA Name: | 8A–14G
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| Gene: | Collagen type VI alpha 1 (COL6A1) |
| Disease: | Ullrich congenital muscular dystrophy (UCMD),Bethlem myopathy (BM) |
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Wild allele/target (5'->3'): | AGAAGCCGGAGAUCCUGGA
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Mutant allele/target (5'->3'): | AGAAGCCAGAGAUGCUGGA
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Target Mutation: | c.850G>A, p.G284R
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Wild siRNA (As strand): | UCUUCGGCCUCUAGGACCU
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ASP-siRNA (As strand): | UCUUCGGUCUCUACGACCU
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Mismatch position in siRNA: | U12C,C6G |
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Mutant allele (5'->3'): | AGAAGCCAGAGAUGCUGGA
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ASP-siRNA (As strand): | UCUUCGGUCUCUACGACCU
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ASP-siRNA efficacy for mutant allele: | 5
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Wild allele (5'->3'): | AGAAGCCGGAGAUCCUGGA
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ASP-siRNA (As strand): | UCUUCGGUCUCUACGACCU
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ASP-siRNA efficacy for wild allele: | 10
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Percentage discrimination for ASP-siRNA: | -5
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Wild allele (5'->3'): | AGAAGCCGGAGAUCCUGGA
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Wild siRNA (As strand): | UCUUCGGCCUCUAGGACCU
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Wild sirna efficacy for wild allele: | 80
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Wild allele (5'->3'): | AGAAGCCGGAGAUCCUGGA
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ASP-siRNA (As strand): | UCUUCGGUCUCUACGACCU
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Wild sirna efficacy for mutant allele: | 40
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Percentage discrimination for wild siRNA: | 40
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| Concentration: | 80nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P12109 |
| Target sequence: | AGGGAGAAGCCGGAGAUCCUGGAA |
| Genbank Accesion: | NM_001848.2 |
| Cell-line: | NIH3T3 |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| OMID ID: | 254090 |
| KEGG disease ID: | H01341 |
| Reference: | 24959844 |
