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siRNA Id: | aspsirna2201
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siRNA Name: | PM4
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| Gene: | Atrophin-1 (ATN-1) |
| Disease: | Dentatorubral-pallidoluysian atrophy (DRPLA) |
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Wild allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Mutant allele/target (5'->3'): | CAGCAGCAUUUUCAGCAGC
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Target Mutation: | c.1462_1464CAG(90_93)
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
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Mismatch position in siRNA: | A8C,A9U,A10G,A11C |
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Mutant allele (5'->3'): | CAGCAGCAUUUUCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
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ASP-siRNA efficacy for mutant allele: | 80
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 50
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
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Wild sirna efficacy for wild allele: | 25
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 5
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| Concentration: | 50nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P54259 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCACG |
| Genbank Accesion: | NM_001007026.1 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Isis Pharmaceuticals |
| Post-transfection duration: | 48 hours |
| OMID ID: | 125370 |
| KEGG disease ID: | K05626 |
| Reference: | 24981774 |
