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siRNA Id: | aspsirna2261
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siRNA Name: | P14
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | ACUUGGGCAAUGUGACUGC
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Mutant allele/target (5'->3'): | ACUUGCGCAAUGUGACUGC
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Target Mutation: | c.256G>C,p.Gly86Arg
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Wild siRNA (As strand): | UGAACCCGUUACACUGACG
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ASP-siRNA (As strand): | UGAACGCGUUACACUGACG
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Mismatch position in siRNA: | G14C |
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Mutant allele (5'->3'): | ACUUGCGCAAUGUGACUGC
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ASP-siRNA (As strand): | UGAACGCGUUACACUGACG
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ASP-siRNA efficacy for mutant allele: | 88
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Wild allele (5'->3'): | ACUUGGGCAAUGUGACUGC
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ASP-siRNA (As strand): | UGAACGCGUUACACUGACG
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ASP-siRNA efficacy for wild allele: | 10
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Percentage discrimination for ASP-siRNA: | 78
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Wild allele (5'->3'): | ACUUGGGCAAUGUGACUGC
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Wild siRNA (As strand): | UGAACCCGUUACACUGACG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | ACUUGGGCAAUGUGACUGC
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ASP-siRNA (As strand): | UGAACGCGUUACACUGACG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | 35
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| Concentration: | 50nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | AGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAGAUGGU |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | Drosophila embryo lysate |
| Experimental technique used: | In vitro cleavage assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 2 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | US2007/0259827A1 |
