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siRNA Id: | aspsirna2369
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siRNA Name: | P10-N10
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | GGAGACUUGGGCAAUGUGA
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Mutant allele/target (5'->3'): | AGUGUAACGGGUUCAGAGG
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Target Mutation: | c.256G>C,p.Gly86Arg
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Wild siRNA (As strand): | CCUCUGAACCCGUUACACU
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ASP-siRNA (As strand): | UCACAUUGCACAAGUCUCC
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Mismatch position in siRNA: | A:G clash |
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Mutant allele (5'->3'): | AGUGUAACGGGUUCAGAGG
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ASP-siRNA (As strand): | UCACAUUGCACAAGUCUCC
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ASP-siRNA efficacy for mutant allele: | NA
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Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
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ASP-siRNA (As strand): | UCACAUUGCACAAGUCUCC
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | 10
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Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
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Wild siRNA (As strand): | CCUCUGAACCCGUUACACU
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
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ASP-siRNA (As strand): | UCACAUUGCACAAGUCUCC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | 20
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| Concentration: | 20nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | AGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAGAUGGU |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 24 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | US2007/0259827A1 |
