| |
|---|
|
siRNA Id: | aspsirna2403
|
|
siRNA Name: | 24
|
| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
|
Wild allele/target (5'->3'): | GUCGUCAGUGUAACGCGUU
|
|
Mutant allele/target (5'->3'): | GUCGUCAGUGUAACGGGUU
|
|
Target Mutation: | c.256G>C,p.Gly86Arg
|
|
Wild siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
ASP-siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
Mismatch position in siRNA: | G16C (G:G clash) |
|
Mutant allele (5'->3'): | GUCGUCAGUGUAACGGGUU
|
|
ASP-siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
ASP-siRNA efficacy for mutant allele: | NA
|
|
Wild allele (5'->3'): | GUCGUCAGUGUAACGCGUU
|
|
ASP-siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
ASP-siRNA efficacy for wild allele: | NA
|
|
Percentage discrimination for ASP-siRNA: | 22
|
|
Wild allele (5'->3'): | GUCGUCAGUGUAACGCGUU
|
|
Wild siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | GUCGUCAGUGUAACGCGUU
|
|
ASP-siRNA (As strand): | CAGCAGUCACAUUGCGCAA
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | 45
|
| Concentration: | 20nM |
| Orientation: | Antisense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | AGGCAUGUUGGAGACUUGGGCAAUGUGACUGCUGACAAAGAUGGU |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 24 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | US2007/0259827A1 |
